Journal: Cell Communication and Signaling : CCS

Article Title: STAT3 blockade ameliorates LPS-induced kidney injury through macrophage-driven inflammation

doi: 10.1186/s12964-024-01841-1

Figure Lengend Snippet: Time-dependent effect of LPS on macrophage subpopulations co-expressed with F4/80 and CD11b. A Schematic diagram of the progression of L-AKI. B Kidney functions were evaluated at various time points following LPS injection in mice: 0, 6, 12, and 24 h ( n = 10 in each group). C Western blot analysis of the expression of pSTAT3, STAT3, and NGAL after LPS injection. D and E Flow cytometry results of immune cell types in the mouse L-AKI model, representing two populations (kidney macrophages, CD11b high F4/80 low and CD11b low F4/80 high ) ( D ); the frequency and ratio of individual populations were quantified using GraphPad Prism ( n = 4 in each group) ( E ). F IHC representative images (left) and quantification (right) of NGAL ( n = 5 in each group), F4/80 ( n = 5 in each group), pSTAT3 ( n = 5 in each group), and PAS from LPS-treated mice. Scale bars, 75 μm (200X), 100 μm (100X), and 50 μm (400X). All experiments were independently replicated at least three times, and the data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: D and E Flow cytometry results of immune cell types in the mouse L-AKI model, representing two populations (kidney macrophages, CD11b high F4/80 low and CD11b low F4/80 high ) ( D ); the frequency and ratio of individual populations were quantified using GraphPad Prism ( n = 4 in each group) ( E ).

Techniques: Injection, Western Blot, Expressing, Flow Cytometry